Catalase and hydrogen peroxide coursework

The oxygen produced in 30 seconds is collected over water. For example, if there were more molecules of yeast, the rate of reaction would increase because there would be more collisions between enzyme and substrate molecules.

Any heavy metal ion such as copper cations in copper II sulfate can act as a noncompetitive inhibitor of catalase.

Another factor which Catalase and hydrogen peroxide coursework hard to measure was the volume of gas produced, because some of the higher concentration reactions were very fast, so it was hard to read the correct values every time.

A stopwatch or any similar device can be used to monitor the time of reaction. This would have affected the number of molecules of hydrogen peroxide present, which in turn would have affected the number of collisions between enzyme and substrate molecules. Catalase enzymes from various species have vastly differing optimum temperatures.

I have drawn a line of best fit to clearly illustrate this trend. Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell. Read up on catalase as a catalyst in this reaction and what factors affect its efficacy.

Try to set out to test as many of these as reasonably possible. For example you are probably going to have to show if a change in temperature affects the efficacy of the enzyme.

All these reactions must be carried out in a temperature controlled environment, at a constant temperature and pH, as per the requirements of the Catalase enzyme for its reaction with hydrogen peroxide for breaking it into hydrogen and oxygen. To prevent this I had to dry out the barrel and syringe before commencing the procedure.

If bubbles form on contact, this indicates a positive catalase result. I tried to make this as accurate as possible by keeping my eyes level with the gas syringe. Investigation - Hydrogen Peroxide Biology Investigation - Hydrogen Peroxide An investigation into how the concentration of the substrate 'hydrogen peroxide' affects the rate at which it is broken down to oxygen and water by the enzyme catalase Hydrogen peroxide is released due to many metabolic reactions in the body, which gives toxicity to the living cells.

I tried to keep this constant by making sure I swirled the conical flask evenly. Thus, the curve plotted in the STEP E can be interpreted with Michaelis-Menton mechanism of enzyme catalysis for studying the effect of concentration of the substrate. Consistent Temperature Temperature is a major factor which affects the rate of reaction.

A stopwatch or any similar device can be used to monitor the time of reaction. Take care when removing the cap of the reagent bottle, as gas pressure may have built up inside.

Investigation - Hydrogen Peroxide An investigation into how the concentration of the substrate 'hydrogen peroxide' affects the rate at which it is broken down to oxygen and water by the enzyme catalase.

Apr 30,  · Catalase is an enzyme which is found in most living organisms. It catalyses the decomposition of hydrogen peroxide into water and oxygen. 2H 2 O 2 + Catalase >>> 2H 2 O + O 2. Catalase dramatically reduces the activation energy needed for the michaelferrisjr.coms: 5. View Answers_to_Labs_Week_4 copy 2 from BSC at Indian River State College.

Answers to Labs Catalase Activity What is the reactant in this reaction? Hydrogen Peroxide What is the substrate94%(17). - The effect of catalase concentration on the breakdown rate of h2o2 The effect of catalase concentrations on the rate of Break down of hydrogen peroxide Scientific knowledge and understanding: A rate is a measure of change that occurs in a given time whilst a reaction is the interaction of substances undergoing chemical change.

Feb 28,  · Hello, I'm doing my GCSE additional science coursework. And I I'm investigating the rate of reaction of the enzyme Catalase and the substrate: Hydrogen Peroxide.

The independent variable I chose to investigate was the Concentration of enzyme i.e. changing the concentration of the enzyme.

We used Yeast as a source of Status: Resolved.

Effect of Substrate Concentration on the Rate of Activity of Catalase

Catalase is an enzyme present in the cells of plants, animals and aerobic (oxygen requiring) bacteria. It promotes the conversion of hydrogen peroxide, a powerful and potentially harmful oxidizing agent, to water and molecular oxygen.

Effect of Substrate Concentration on the Rate of Activity of Catalase Catalase and hydrogen peroxide coursework
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